PXD013274-1

PXD013274 is an original dataset announced via ProteomeXchange.

Title	The beta-hydroxyaspartate cycle is used for glycolate assimilation by ubiquitous marine Proteobacteria
Description	Annual net primary production in global oceans is estimated at 50 petagrams (50 * 1015 g) of carbon1. The metabolite glycolate is commonly secreted by marine phytoplankton as a side product of photosynthesis, resulting in an estimated annual flux of one petagram2, making it an abundant carbon source in the ocean. Here we show Here we show that ubiquitous marine Proteobacteria assimilate 23 glyoxylate, the oxidation product of glycolate, via the beta-hydroxyaspartate cycle (BHAC). This cycle represents the most efficient route for glyoxylate assimilation described to date, allowing for the direct conversion of this C2 compound into the tricarboxylic acid cycle intermediate oxaloacetate. We identified the gene cluster encoding for the BHAC and characterized its four enzymes, including a previously unknown primary imine reductase. Furthermore, we determined crystal structures of the two key enzymes of the BHAC, investigated the phylogenetic distribution of the pathway and demonstrated presence and active expression of its genes during phytoplankton blooms in the North Sea. Our results add a new facet to the trophic interactions of photosynthetic primary producers and heterotrophic bacterioplankton in the global oceans, closing an overlooked gap in the marine carbon cycle. This proteomic data set compares the Paracoccus denitrificans proteome under glyoxycolate and succinate growth.
HostingRepository	PRIDE
AnnounceDate	2019-08-14
AnnouncementXML	Submission_2019-08-14_04:42:09.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013274
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Paracoccus denitrificans PD1222; NCBI TaxID: 318586;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-28 01:10:21	ID requested
⏵ 1	2019-08-14 04:42:10	announced
2	2019-10-11 05:40:01	announced	2019-10-11: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Paracoccus denitrificans

Tobias Juergen Erb
contact affiliation	Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for terrestrial Microbiology, Marburg, Germany
contact email	toerb@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013274

PRIDE project URI

• PRIDE
  1. PXD013274
     1. Label: PRIDE project
     2. Name: The beta-hydroxyaspartate cycle is used for glycolate assimilation by ubiquitous marine Proteobacteria