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PXD013185-1

PXD013185 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpecies-specific quantitative proteomics profiles of sarcoma patient-derived models closely reflect their primary tumors 
DescriptionAvailability of patient-derived sarcoma models that closely mimic human tumors remains a significant gap in cancer research as these models may not recapitulate the spectrum of sarcoma heterogeneity seen in patients. To characterize patient-derived models for functional studies, we made proteomic comparisons with originating sarcomas representative of the three intrinsic subtypes by mass spectrometry. Human protein profiling was found to be retained with high fidelity in patient-derived models. Patient derived xenografts locally invade and colonize stroma in mice which enables unambiguous molecular discrimination of human proteins in the tumor from mouse proteins in the microenvironment. We characterized protein profiling of patient sarcoma tumors and mouse stroma by species-specific quantitative proteomics. We found that protein expression in mouse stroma was affected by the primary human tumor. Our results showed that levels of stromal proteins derived from the tumor were lowered in PDXs and cell lines and part of human stromal proteins were replaced by corresponding mouse proteins in PDXs. This suggests that the effects of the microenvironment on drug response may not reflect those in the primary tumor. This cross-species proteomic analysis in PDXs could potentially improve preclinical evaluation of treatment modalities and enhance the ability to predict clinical trial responses.
HostingRepositoryjPOST
AnnounceDate2020-03-22
AnnouncementXMLSubmission_2020-03-21_08:00:11.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKumiko Shiozawa
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxymethyl-L-cysteine; L-methionine sulfoxide
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-21 22:42:53ID requested
12020-03-21 08:00:12announced
22021-02-04 16:47:14announced2021-02-04: Updated FTP location.
32022-09-18 02:29:36announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteome
Patient-derived models
Sarcoma
Contact List
Tadashi Kondo
lab head
Kumiko Shiozawa
contact affiliationNational Cancer Centre
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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