PXD013113 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes |
Description | HaCaT, and its three retrovirally c-Ha-ras-transformed subclones, A5, II-4 and RT3 (abbreviated as ras-HaCaTs in the text) that exhibit benign, invasive and malignant phenotypes, respectively, were cultured as 3D monocultures and co-cultures with fibroblasts. After hypotonic lysis of the cells, the insoluble proteins were analysed by LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-11 |
AnnouncementXML | Submission_2019-10-30_02:13:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pekka Rappu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-18 02:50:42 | ID requested | |
1 | 2019-09-11 00:58:22 | announced | |
⏵ 2 | 2019-10-30 02:13:44 | announced | 2019-10-30: Updated publication reference for PubMed record(s): 31655292. |
Publication List
Siljam, ä, ki E, Rappu P, Riihil, ä P, Nissinen L, K, ä, h, ä, ri VM, Heino J, signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma. Matrix Biol, 87():26-47(2020) [pubmed] |
Keyword List
submitter keyword: HRAS, HaCat, fibroblast keratinocyte |
Contact List
Jyrki Heino |
contact affiliation | Biochemistry, University of Turku, Turku, Finland |
contact email | jyrki.heino@utu.fi |
lab head | |
Pekka Rappu |
contact affiliation | University of Turku |
contact email | pekka.rappu@utu.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013113 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013113
- Label: PRIDE project
- Name: Proteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes