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PXD013113 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes
DescriptionHaCaT, and its three retrovirally c-Ha-ras-transformed subclones, A5, II-4 and RT3 (abbreviated as ras-HaCaTs in the text) that exhibit benign, invasive and malignant phenotypes, respectively, were cultured as 3D monocultures and co-cultures with fibroblasts. After hypotonic lysis of the cells, the insoluble proteins were analysed by LC-MS/MS.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPekka Rappu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-18 02:50:42ID requested
12019-09-11 00:58:22announced
22019-10-30 02:13:44announced2019-10-30: Updated publication reference for PubMed record(s): 31655292.
Publication List
Siljamäki E, Rappu P, Riihilä P, Nissinen L, Kähäri VM, Heino J, H-Ras activation and fibroblast-induced TGF-β signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma. Matrix Biol, 87():26-47(2020) [pubmed]
Keyword List
submitter keyword: HRAS, HaCat, fibroblast keratinocyte
Contact List
Jyrki Heino
contact affiliationBiochemistry, University of Turku, Turku, Finland
contact emailjyrki.heino@utu.fi
lab head
Pekka Rappu
contact affiliationUniversity of Turku
contact emailpekka.rappu@utu.fi
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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