PXD012985-1

PXD012985 is an original dataset announced via ProteomeXchange.

Title	Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B
Description	Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptome level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrosarcoma cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuable resource in understanding the processes that are occuring during cytokine stimulation of chondrocytic cells.
HostingRepository	PRIDE
AnnounceDate	2019-07-11
AnnouncementXML	Submission_2019-07-11_04:22:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012985
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ben McDermott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-05 08:49:50	ID requested
⏵ 1	2019-07-11 04:30:52	announced
2	2019-08-19 04:29:58	announced	Updated publication reference for PubMed record(s): 31300557.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Human, chondrocytes, ribosome profiling, interleukin-1B, inflammation, secretion

Dr Simon Tew
contact affiliation	Comparative Musculoskeletal Sciences Research Group Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool
contact email	Tew@liverpool.ac.uk
lab head
Ben McDermott
contact affiliation	University of Liverpool
contact email	benmcd@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012985
     1. Label: PRIDE project
     2. Name: Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B