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PXD012985

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-07-11
  • AnnouncementXML: Submission_2019-08-19_04:29:57.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD012985
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Ben McDermott
  • Title: Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B
  • Description: Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptome level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrosarcoma cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuable resource in understanding the processes that are occuring during cytokine stimulation of chondrocytic cells.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: Oxidation; Carbamidomethyl
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-03-05 08:49:50ID requested
12019-07-11 04:30:52announced
22019-08-19 04:29:58announcedUpdated publication reference for PubMed record(s): 31300557.

Publication List

  1. McDermott BT, Peffers MJ, McDonagh B, Tew SR, Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1β. J Biol Chem, ():(2019) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: Human, chondrocytes, ribosome profiling, interleukin-1B, inflammation, secretion

Contact List

    Dr Simon Tew
    • contact affiliation: Comparative Musculoskeletal Sciences Research Group Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool
    • contact email: Tew@liverpool.ac.uk
    • lab head:
    Ben McDermott
    • contact affiliation: University of Liverpool
    • contact email: benmcd@liverpool.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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