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PXD012985 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranslational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B
DescriptionChondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptome level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrosarcoma cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuable resource in understanding the processes that are occuring during cytokine stimulation of chondrocytic cells.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBen McDermott
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-05 08:49:50ID requested
12019-07-11 04:30:52announced
22019-08-19 04:29:58announcedUpdated publication reference for PubMed record(s): 31300557.
Publication List
McDermott BT, Peffers MJ, McDonagh B, Tew SR, Translational regulation contributes to the secretory response of chondrocytic cells following exposure to interleukin-1β. J Biol Chem, 294(35):13027-13039(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, chondrocytes, ribosome profiling, interleukin-1B, inflammation, secretion
Contact List
Dr Simon Tew
contact affiliationComparative Musculoskeletal Sciences Research Group Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool
contact emailTew@liverpool.ac.uk
lab head
Ben McDermott
contact affiliationUniversity of Liverpool
contact emailbenmcd@liverpool.ac.uk
dataset submitter
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