PXD012985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B |
Description | Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptome level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrosarcoma cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuable resource in understanding the processes that are occuring during cytokine stimulation of chondrocytic cells. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-11 |
AnnouncementXML | Submission_2019-08-19_04:29:57.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD012985 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ben McDermott |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-05 08:49:50 | ID requested | |
1 | 2019-07-11 04:30:52 | announced | |
⏵ 2 | 2019-08-19 04:29:58 | announced | Updated publication reference for PubMed record(s): 31300557. |
Publication List
McDermott BT, Peffers MJ, McDonagh B, Tew SR, . J Biol Chem, 294(35):13027-13039(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, chondrocytes, ribosome profiling, interleukin-1B, inflammation, secretion |
Contact List
Dr Simon Tew |
contact affiliation | Comparative Musculoskeletal Sciences Research Group Department of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool |
contact email | Tew@liverpool.ac.uk |
lab head | |
Ben McDermott |
contact affiliation | University of Liverpool |
contact email | benmcd@liverpool.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012985
- Label: PRIDE project
- Name: Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B