PXD012930 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha |
Description | Estrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used to pull down the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06-935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-21 |
AnnouncementXML | Submission_2019-04-18_03:37:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Evangelia Papachristou |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-01 04:02:49 | ID requested | |
1 | 2019-03-21 04:06:50 | announced | |
⏵ 2 | 2019-04-18 03:47:24 | announced | Updated publication reference for PubMed record(s): 30970003. |
Publication List
Glont SE, Papachristou EK, Sawle A, Holmes KA, Carroll JS, Siersbaek R, Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha. PLoS One, 14(4):e0215340(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: estrogen receptor alpha, ChIP-seq, RIME |
Contact List
Jason Carroll |
contact affiliation | Senior Group Leader |
contact email | Jason.Carroll@cruk.cam.ac.uk |
lab head | |
Evangelia Papachristou |
contact affiliation | Senior Scientific Assistant |
contact email | Eva.Papachristou@cruk.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012930
- Label: PRIDE project
- Name: Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha