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PXD012930

PXD012930 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha
DescriptionEstrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used to pull down the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06-935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments.
HostingRepositoryPRIDE
AnnounceDate2019-03-21
AnnouncementXMLSubmission_2019-04-18_03:37:11.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEvangelia Papachristou
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-01 04:02:49ID requested
12019-03-21 04:06:50announced
22019-04-18 03:47:24announcedUpdated publication reference for PubMed record(s): 30970003.
Publication List
Glont SE, Papachristou EK, Sawle A, Holmes KA, Carroll JS, Siersbaek R, Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha. PLoS One, 14(4):e0215340(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: estrogen receptor alpha, ChIP-seq, RIME
Contact List
Jason Carroll
contact affiliationSenior Group Leader
contact emailJason.Carroll@cruk.cam.ac.uk
lab head
Evangelia Papachristou
contact affiliationSenior Scientific Assistant
contact emailEva.Papachristou@cruk.cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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