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PXD012925-1

PXD012925 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate the emergence of 2C-like cells and zygotic transcriptional program
DescriptionThe molecular mechanism controlling the zygotic genome activation (ZGA) in mammals remains poorly understood. The 2C-like cells spontaneously emerging from cultures of mouse embryonic stem cells (ESCs) share some key transcriptional and epigenetic programs with 2-cell stage embryos. By studying the transition of ESCs into 2C-like cells, we identified Dppa2/4 as important regulators controlling zygotic transcriptional program through directly upregulating the expression of Dux. In addition, we found that DPPA2 protein is sumoylated and its activity is negatively regulated by Sumo E3 ligase PIAS4. PIAS4 is downregulated during zygotic genome activation process and during transitioning of ESCs into 2C-like cells. Depleting Pias4 or overexpressing Dppa2/4 is sufficient to upregulateactivate 2C-like transcriptional program, while depleting Dppa2/4 or forced expression of PIAS4 or Sumo2-Dppa2 inhibits 2C-like transcriptional program. Furthermore, ectopic expression of Pias4 or Sumo2-Dppa2 impairs early mouse embryo development. In summary, our study identifies key molecular rivals consisting of transcription factors and a Sumo2 E3 ligase that regulate the transition of ESCs into 2C-like cells and zygotic transcriptional program upstream of Dux.
HostingRepositoryPRIDE
AnnounceDate2019-03-06
AnnouncementXMLSubmission_2019-03-06_00:45:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYangming Wang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-01 02:57:17ID requested
12019-03-06 00:45:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Pias4 Dppa2 ZGA Sumoylation
Contact List
Yangming Wang
contact affiliationLab of Stem cells adn noncoding RNAs, Institue of Molecular Medicine, Peking University
contact emailyangming.wang@pku.edu.cn
lab head
Yangming Wang
contact affiliationPeking University
contact emailyangming.wang@pku.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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