PXD012809 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interaction networks of Weibel-Palade body regulators syntaxin-3 and syntaxin binding protein 5 in endothelial cells |
Description | The endothelium stores the hemostatic protein Von Willebrand factor (VWF) in endothelial storage organelles called Weibel-Palade bodies (WPBs). During maturation, WPBs recruit a complex of Rab GTPases and effectors that associate with components of the SNARE machinery that control WPB exocytosis. Recent genome wide association studies have found links between genetic variations in the SNAREs syntaxin-2 (STX2) and syntaxin binding protein 5 (STXBP5) and VWF plasma levels, suggesting a role for SNARE proteins in regulating VWF release. Moreover, we have previously identified the SNARE proteins syntaxin-3 and STXBP1 as regulators of WPB release. In this study we used an unbiased iterative interactomic approach to identify new components of the WPB exocytotic machinery. An interactome screen of syntaxin-3 identifies a number of SNAREs and SNARE associated proteins (STXBP2, STXBP5, SNAP23, NAPA and NSF). We show that the VAMP-like domain (VLD) of STXBP5 is indispensable for the interaction with SNARE proteins and this capacity of the VLD could be exploited to identify an extended set of novel endothelial SNARE interactors of STXBP5. In addition, an STXBP5 variant with an N436S substitution, which is linked to lower VWF plasma levels, does not show a difference in interactome when compared with WT STXBP5. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-06 |
AnnouncementXML | Submission_2023-01-06_08:17:25.070.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | RubenBierings |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-21 01:12:58 | ID requested | |
⏵ 1 | 2023-01-06 08:17:26 | announced | |
2 | 2023-11-14 07:31:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Schillemans M, Karampini E, Hoogendijk AJ, Wahedi M, van Alphen FPJ, van den Biggelaar M, Voorberg J, Bierings R, Interaction networks of Weibel-Palade body regulators syntaxin-3 and syntaxin binding protein 5 in endothelial cells. J Proteomics, 205():103417(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Weibel-Palade body |
SNARE protein |
syntaxin |
protein-protein interactions |
Contact List
RubenBierings |
contact affiliation | Molecular and Cellular Hemostasis and 2Research Facilities, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, The Netherlands Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands. |
contact email | r.bierings@erasmusmc.nl |
lab head | |
RubenBierings |
contact affiliation | ErasmusMC |
contact email | r.bierings@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012809
- Label: PRIDE project
- Name: Interaction networks of Weibel-Palade body regulators syntaxin-3 and syntaxin binding protein 5 in endothelial cells