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PXD012800-2

PXD012800 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOn-chip AP-MS: Interaction proteomics on a microfluidics platform
DescriptionEssentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin, CCC and Mediator complex from as little as 4 micrograms of input lysate, representing a 50─100-fold downscaling compared to regular microcentrifuge tube-based protocols. We show that our platform can be used to affinity purify tagged baits as well as native cellular proteins and their interaction partners. As such, our method holds great promise for future biological and clinical AP-MS applications in which sample amounts are limited.
HostingRepositoryPRIDE
AnnounceDate2019-04-02
AnnouncementXMLSubmission_2019-05-14_07:19:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCristina Furlan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-20 06:46:23ID requested
12019-04-02 08:28:11announced
22019-05-14 07:19:17announcedUpdated publication reference for PubMed record(s): 30948724.
Publication List
Furlan C, Dirks RAM, Thomas PC, Jones RC, Wang J, Lynch M, Marks H, Vermeulen M, Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements. Nat Commun, 10(1):1525(2019) [pubmed]
Keyword List
submitter keyword: AP-MS, low input, interaction proteomics
Contact List
Michiel Vermeulen
contact affiliationDepartment of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands
contact emailMichiel.Vermeulen@science.ru.nl
lab head
Cristina Furlan
contact affiliationRadboud University
contact emailc.furlan@science.ru.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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