PXD012800

PXD012800 is an original dataset announced via ProteomeXchange.

Title	On-chip AP-MS: Interaction proteomics on a microfluidics platform
Description	Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin, CCC and Mediator complex from as little as 4 micrograms of input lysate, representing a 50─100-fold downscaling compared to regular microcentrifuge tube-based protocols. We show that our platform can be used to affinity purify tagged baits as well as native cellular proteins and their interaction partners. As such, our method holds great promise for future biological and clinical AP-MS applications in which sample amounts are limited.
HostingRepository	PRIDE
AnnounceDate	2019-04-02
AnnouncementXML	Submission_2019-05-14_07:19:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Furlan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-20 06:46:23	ID requested
1	2019-04-02 08:28:11	announced
⏵ 2	2019-05-14 07:19:17	announced	Updated publication reference for PubMed record(s): 30948724.

Furlan C, Dirks RAM, Thomas PC, Jones RC, Wang J, Lynch M, Marks H, Vermeulen M, Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements. Nat Commun, 10(1):1525(2019) [pubmed]

submitter keyword: AP-MS, low input, interaction proteomics

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands
contact email	Michiel.Vermeulen@science.ru.nl
lab head
Cristina Furlan
contact affiliation	Radboud University
contact email	c.furlan@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012800

PRIDE project URI

• PRIDE
  1. PXD012800
     1. Label: PRIDE project
     2. Name: On-chip AP-MS: Interaction proteomics on a microfluidics platform