PXD012790-1

PXD012790 is an original dataset announced via ProteomeXchange.

Title	b-JIM regulates the function of chromosomal kinase JIL-1 at H3K36me3 chromatin
Description	In flies, the essential kinase JIL-1 responsible for most interphase H3S10ph is stabilized and targeted to chromatin by a new PWWP domain containing protein, which we called b-JIM. b-JIM binds JIL-1 within the conserved C-terminal domain which is still present Su(var)3-1 alleles of the JIL-1 gene and this binding is essential for the stability of JIL-1. The b-JIM/JIL-1 (JJ) complex is the major form of the kinase in vivo and interacts with other complexes implicated in the regulation of transcription. The most prominent new interactors, Dpy30L1 and BOD1 probably mediate the interaction with the Set1/COMPASS complex. We show that the JJ complex directly binds to H3K36me3 nucleosomes via the PWWP domain of b-JIM, and that this is the main mode of recruitment of JIL-1 to chromatin in vivo. Upon knock down of JIL-1 or the JJ complex, transcript levels from X-linked genes and many transposons are most prominently affected in male cells.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_08:24:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-19 05:34:06	ID requested
⏵ 1	2019-09-27 08:24:07	announced
2	2019-12-11 06:56:00	announced	2019-12-11: Updated publication reference for PubMed record(s): 31767855.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: JIL-1 , b-JIM , PWWP , H3S10ph , H3K36me3 , chromatin , BOD1 , Dpy30-L1, Set1, Compass

Dr. Catherine Regnard
contact affiliation	Biomedical Center Munich Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried Phone: (089) 2180 71602
contact email	catherine.regnard@med.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD012790

PRIDE project URI

• PRIDE
  1. PXD012790
     1. Label: PRIDE project
     2. Name: b-JIM regulates the function of chromosomal kinase JIL-1 at H3K36me3 chromatin