PXD012790

PXD012790 is an original dataset announced via ProteomeXchange.

Title	JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
Description	In Drosophila the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, like dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). The JASPer-JIL-1 (JJ)-complex is the major form of the kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, where the complex modulates the transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify several interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.
HostingRepository	PRIDE
AnnounceDate	2019-10-09
AnnouncementXML	Submission_2019-12-11_06:55:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-19 05:34:06	ID requested
1	2019-09-27 08:24:07	announced
⏵ 2	2019-12-11 06:56:00	announced	2019-12-11: Updated publication reference for PubMed record(s): 31767855.

Albig C, Wang C, Dann GP, Wojcik F, Schauer T, Krause S, Maenner S, Cai W, Li Y, Girton J, Muir TW, Johansen J, Johansen KM, Becker PB, Regnard C, JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila. Nat Commun, 10(1):5343(2019) [pubmed]

curator keyword: Biological

submitter keyword: JIL-1 , b-JIM , PWWP , H3S10ph , H3K36me3 , chromatin , BOD1 , Dpy30-L1, Set1, Compass

Dr. Catherine Regnard
contact affiliation	Biomedical Center Munich Molecular Biology Großhaderner Strasse 9 82152 Planegg-Martinsried Phone: (089) 2180 71602
contact email	catherine.regnard@med.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD012790

PRIDE project URI

• PRIDE
  1. PXD012790
     1. Label: PRIDE project
     2. Name: JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila