PXD012697-1
PXD012697 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Exploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway |
Description | Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in E. coli to produce several novel terpenes containing sixteen carbons. Changing the terpene cyclase generated different novel terpene product profiles. To further validate the new compounds we confirmed 13C propionate was incorporated, and that the masses and fragmentation patterns were consistent with novel sixteen carbon terpenes by GC-QTOF. Based on the available FPP analogues lepidoptera produce, this approach should greatly expand the reachable biochemical space with applications in areas where terpenes have traditionally found uses. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2019-10-29 |
AnnouncementXML | Submission_2019-10-29_08:46:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Chris Petzold |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6460 Triple Quadrupole LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-02-13 17:19:18 | ID requested | |
⏵ 1 | 2019-10-29 08:46:37 | announced |
Publication List
Eiben CB, de Rond T, Bloszies C, Gin J, Chiniquy J, Baidoo EEK, Petzold CJ, Hillson NJ, Fiehn O, Keasling JD, Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production. ACS Synth Biol, 8(10):2238-2247(2019) [pubmed] |
Keyword List
submitter keyword: Mevalonate Pathway, Terpenes, Juvenile Hormones, homoisopentenyl pyrophosphate, HIPP, homodimethylallyl pyrophosphate, HDMAPP |
Contact List
Chris Petzold | |
---|---|
contact affiliation | Lawrence Berkeley National Laboratory |
contact email | cjpetzold@lbl.gov |
lab head | |
Chris Petzold | |
contact affiliation | Lawrence Berkeley National Laboratory |
contact email | cjpetzold@lbl.gov |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |