PXD012697

PXD012697 is an original dataset announced via ProteomeXchange.

Title	Exploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway
Description	Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in E. coli to produce several novel terpenes containing sixteen carbons. Changing the terpene cyclase generated different novel terpene product profiles. To further validate the new compounds we confirmed 13C propionate was incorporated, and that the masses and fragmentation patterns were consistent with novel sixteen carbon terpenes by GC-QTOF. Based on the available FPP analogues lepidoptera produce, this approach should greatly expand the reachable biochemical space with applications in areas where terpenes have traditionally found uses.
HostingRepository	PanoramaPublic
AnnounceDate	2019-10-29
AnnouncementXML	Submission_2019-10-29_08:46:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chris Petzold
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-13 17:19:18	ID requested
⏵ 1	2019-10-29 08:46:37	announced

Eiben CB, de Rond T, Bloszies C, Gin J, Chiniquy J, Baidoo EEK, Petzold CJ, Hillson NJ, Fiehn O, Keasling JD, Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production. ACS Synth Biol, 8(10):2238-2247(2019) [pubmed]

submitter keyword: Mevalonate Pathway, Terpenes, Juvenile Hormones, homoisopentenyl pyrophosphate, HIPP, homodimethylallyl pyrophosphate, HDMAPP

Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
lab head
Chris Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Panorama Public dataset URI