PXD012433-4
PXD012433 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Simulated spectral library searching on TripleTOF phosphopeptide datasets |
Description | Synthetic phosphopeptides and enriched K562 cell phosphopeptides were analyzed using a TripleTOF 6600 mass spectrometer before and after enzymatic dephosphorylation. Dephosphorylated peptides identified by X!Tandem database searching were subjected to spectral simulation of all possible single phosphorylations using SimPhospho software. Phosphopeptides were identified and localized by SpectraST searching against a library of the simulated spectra, and also by MaxQuant database searching. |
HostingRepository | jPOST |
AnnounceDate | 2019-12-03 |
AnnouncementXML | Submission_2022-09-18_03:33:25.712.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Susumu Y. Imanishi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-01-19 05:51:28 | ID requested | |
1 | 2019-12-02 15:23:58 | announced | |
2 | 2019-12-04 02:53:28 | announced | 2019-12-04: Updated PubMed. |
3 | 2021-02-04 17:12:37 | announced | 2021-02-04: Updated FTP location. |
⏵ 4 | 2022-09-18 03:33:26 | announced | 2022-09-18: Updated FTP location. |
Publication List
Takai A, Tsubosaka T, Hirano Y, Hayakawa N, Tani F, Haapaniemi P, Suni V, Imanishi SY, Optimization of TripleTOF spectral simulation and library searching for confident localization of phosphorylation sites. PLoS One, 14(12):e0225885(2019) [pubmed] |
Keyword List
submitter keyword: Simulated phosphopeptides, SpectraST, TripleTOF |
Contact List
Susumu Y. Imanishi | |
---|---|
lab head | |
Susumu Y. Imanishi | |
contact affiliation | Meijo University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000548/ |