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PXD012433

PXD012433 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSimulated spectral library searching on TripleTOF phosphopeptide datasets
DescriptionSynthetic phosphopeptides and enriched K562 cell phosphopeptides were analyzed using a TripleTOF 6600 mass spectrometer before and after enzymatic dephosphorylation. Dephosphorylated peptides identified by X!Tandem database searching were subjected to spectral simulation of all possible single phosphorylations using SimPhospho software. Phosphopeptides were identified and localized by SpectraST searching against a library of the simulated spectra, and also by MaxQuant database searching.
HostingRepositoryjPOST
AnnounceDate2019-12-03
AnnouncementXMLSubmission_2019-12-04_02:53:27.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusumu Y. Imanishi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-19 05:51:28ID requested
12019-12-02 15:23:58announced
22019-12-04 02:53:28announced2019-12-04: Updated PubMed.
Publication List
Takai A, Tsubosaka T, Hirano Y, Hayakawa N, Tani F, Haapaniemi P, Suni V, Imanishi SY, Optimization of TripleTOF spectral simulation and library searching for confident localization of phosphorylation sites. PLoS One, 14(12):e0225885(2019) [pubmed]
Keyword List
submitter keyword: Simulated phosphopeptides, SpectraST, TripleTOF
Contact List
Susumu Y. Imanishi
lab head
Susumu Y. Imanishi
contact affiliationMeijo University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location