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PXD012390 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-terminal amino acid identification using targeted mass spectrometry
DescriptionUsing engineered initiator tRNA for precise control of protein translation within cells has great promise within future orthogonal translation systems to decouple housekeeping protein metabolism from that of engineered genetic systems. Previously, E. coli strain C321.ΔA.exp lacking all UAG stop codons was created, freeing this ‘amber’ stop codon for other purposes. An engineered ‘amber initiator’ that activates translation at UAG codons is available, but little is known about amino acid it assigns to the amber start codon. Here, we combine for the first time the amber initiator tRNA in C321.ΔA.exp and measure its cellular effects. Using, targeted proteomics, we found that the amber initiator tRNA initiates proteins exclusively with methionine.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRussel Vincent
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListAcetyl; Gln->pyro-Glu; Carboxymethyl; Carbamidomethyl; Oxidation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-16 14:20:31ID requested
12019-03-15 08:58:53announced
Publication List
Vincent RM, Wright BW, Jaschke PR, Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism. ACS Synth Biol, 8(4):675-685(2019) [pubmed]
Keyword List
submitter keyword: Parallel reaction monitoring, amber initiator tRNA, nanoluciferase, N-terminal amino acid
Contact List
Paul Jaschke
contact affiliationMacquarie University
contact emailpaul.jaschke@mq.edu.au
lab head
Russel Vincent
contact affiliationMacquarie University
contact emailrussel-miranda.vincent@students.mq.edu.au
dataset submitter
Full Dataset Link List
Panorama Public dataset URI