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PXD012390

DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-03-15
  • AnnouncementXML: Submission_2019-03-15_08:58:52.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Russel Vincent
  • Title: N-terminal amino acid identification using targeted mass spectrometry
  • Description: Using engineered initiator tRNA for precise control of protein translation within cells has great promise within future orthogonal translation systems to decouple housekeeping protein metabolism from that of engineered genetic systems. Previously, E. coli strain C321.ΔA.exp lacking all UAG stop codons was created, freeing this ‘amber’ stop codon for other purposes. An engineered ‘amber initiator’ that activates translation at UAG codons is available, but little is known about amino acid it assigns to the amber start codon. Here, we combine for the first time the amber initiator tRNA in C321.ΔA.exp and measure its cellular effects. Using, targeted proteomics, we found that the amber initiator tRNA initiates proteins exclusively with methionine.
  • SpeciesList: scientific name: Escherichia coli; NCBI TaxID: 562;
  • ModificationList: Acetyl; Gln->pyro-Glu; Carboxymethyl; Carbamidomethyl; Oxidation
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-01-16 14:20:31ID requested
12019-03-15 08:58:53announced

Publication List

  1. Vincent RM, Wright BW, Jaschke PR, Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism. ACS Synth Biol, 8(4):675-685(2019) [pubmed]

Keyword List

  1. submitter keyword: Parallel reaction monitoring, amber initiator tRNA, nanoluciferase, N-terminal amino acid

Contact List

    Paul Jaschke
    • contact affiliation: Macquarie University
    • contact email: paul.jaschke@mq.edu.au
    • lab head:
    Russel Vincent
    • contact affiliation: Macquarie University
    • contact email: russel-miranda.vincent@students.mq.edu.au
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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