PXD012390

PXD012390 is an original dataset announced via ProteomeXchange.

Title	N-terminal amino acid identification using targeted mass spectrometry
Description	Using engineered initiator tRNA for precise control of protein translation within cells has great promise within future orthogonal translation systems to decouple housekeeping protein metabolism from that of engineered genetic systems. Previously, E. coli strain C321.ΔA.exp lacking all UAG stop codons was created, freeing this ‘amber’ stop codon for other purposes. An engineered ‘amber initiator’ that activates translation at UAG codons is available, but little is known about amino acid it assigns to the amber start codon. Here, we combine for the first time the amber initiator tRNA in C321.ΔA.exp and measure its cellular effects. Using, targeted proteomics, we found that the amber initiator tRNA initiates proteins exclusively with methionine.
HostingRepository	PanoramaPublic
AnnounceDate	2019-03-15
AnnouncementXML	Submission_2019-03-15_08:58:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Russel Vincent
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Acetyl; Gln->pyro-Glu; Carboxymethyl; Carbamidomethyl; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 14:20:31	ID requested
⏵ 1	2019-03-15 08:58:53	announced

Vincent RM, Wright BW, Jaschke PR, Measuring Amber Initiator tRNA Orthogonality in a Genomically Recoded Organism. ACS Synth Biol, 8(4):675-685(2019) [pubmed]

submitter keyword: Parallel reaction monitoring, amber initiator tRNA, nanoluciferase, N-terminal amino acid

Paul Jaschke
contact affiliation	Macquarie University
contact email	paul.jaschke@mq.edu.au
lab head
Russel Vincent
contact affiliation	Macquarie University
contact email	russel-miranda.vincent@students.mq.edu.au
dataset submitter

Panorama Public dataset URI