PXD012372-1

PXD012372 is an original dataset announced via ProteomeXchange.

Title	 LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins
Description	In the germline nucleus of Tetrahymena and other ciliates there occurs a peculiar burst of transcriptional activity during sexual reproduction. A major part of the genome is transcribed into noncoding RNA (ncRNA). This ncRNA serves to spot transposable elements (TEs) and other unwanted DNA sequences and marks them for elimination from the soma of the progeny. ncRNA transcription is remarkable for its extent, its occurrence in a nucleus that bears heterochromatic marks and is incapable of transcribing mRNAs and for its concomitance with meiotic DNA recombination. Here we report two novel factors (Emit1 and Emit2) that promote the micronuclear localization of the transcription Mediator complex to allow this unconventional transcriptional program to occur. Another factor (Rib1) may enhance the transcription activity to TE-rich pericentromeric and telomeric regions. The purpose of the LC-MS/MS analysis of Emit1, Emit2 and Rib1 immunoprecipitation samples is to identify their potential interacting partner proteins.
HostingRepository	PRIDE
AnnounceDate	2019-07-11
AnnouncementXML	Submission_2019-07-11_00:08:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao Tian
SpeciesList	scientific name: Tetrahymena thermophila SB210; NCBI TaxID: 312017;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-16 02:28:21	ID requested
⏵ 1	2019-07-11 00:08:35	announced

Tian M, Mochizuki K, Loidl J, Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins. Curr Biol, 29(14):2359-2370.e5(2019) [pubmed]

curator keyword: Biological

submitter keyword: Tetrahymena thermophila, Mediator complex, Emit1, Emit2, Rib1

Josef Loidl
contact affiliation	Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Austria
contact email	josef.loidl@univie.ac.at
lab head
Miao Tian
contact affiliation	Max F. Perutz Laboratories
contact email	tian.miao@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012372
     1. Label: PRIDE project
     2. Name:  LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins