PXD012372 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins |
Description | In the germline nucleus of Tetrahymena and other ciliates there occurs a peculiar burst of transcriptional activity during sexual reproduction. A major part of the genome is transcribed into noncoding RNA (ncRNA). This ncRNA serves to spot transposable elements (TEs) and other unwanted DNA sequences and marks them for elimination from the soma of the progeny. ncRNA transcription is remarkable for its extent, its occurrence in a nucleus that bears heterochromatic marks and is incapable of transcribing mRNAs and for its concomitance with meiotic DNA recombination. Here we report two novel factors (Emit1 and Emit2) that promote the micronuclear localization of the transcription Mediator complex to allow this unconventional transcriptional program to occur. Another factor (Rib1) may enhance the transcription activity to TE-rich pericentromeric and telomeric regions. The purpose of the LC-MS/MS analysis of Emit1, Emit2 and Rib1 immunoprecipitation samples is to identify their potential interacting partner proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-11 |
AnnouncementXML | Submission_2019-07-11_00:08:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miao Tian |
SpeciesList | scientific name: Tetrahymena thermophila SB210; NCBI TaxID: 312017; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-16 02:28:21 | ID requested | |
⏵ 1 | 2019-07-11 00:08:35 | announced | |
Publication List
Tian M, Mochizuki K, Loidl J, Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins. Curr Biol, 29(14):2359-2370.e5(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Tetrahymena thermophila, Mediator complex, Emit1, Emit2, Rib1 |
Contact List
Josef Loidl |
contact affiliation | Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Austria |
contact email | josef.loidl@univie.ac.at |
lab head | |
Miao Tian |
contact affiliation | Max F. Perutz Laboratories |
contact email | tian.miao@univie.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012372
- Label: PRIDE project
- Name: LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins