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PXD012372

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-07-11
  • AnnouncementXML: Submission_2019-07-11_00:08:34.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Miao Tian
  • Title:  LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins
  • Description: In the germline nucleus of Tetrahymena and other ciliates there occurs a peculiar burst of transcriptional activity during sexual reproduction. A major part of the genome is transcribed into noncoding RNA (ncRNA). This ncRNA serves to spot transposable elements (TEs) and other unwanted DNA sequences and marks them for elimination from the soma of the progeny. ncRNA transcription is remarkable for its extent, its occurrence in a nucleus that bears heterochromatic marks and is incapable of transcribing mRNAs and for its concomitance with meiotic DNA recombination. Here we report two novel factors (Emit1 and Emit2) that promote the micronuclear localization of the transcription Mediator complex to allow this unconventional transcriptional program to occur. Another factor (Rib1) may enhance the transcription activity to TE-rich pericentromeric and telomeric regions. The purpose of the LC-MS/MS analysis of Emit1, Emit2 and Rib1 immunoprecipitation samples is to identify their potential interacting partner proteins.
  • SpeciesList: scientific name: Tetrahymena thermophila SB210; NCBI TaxID: 312017;
  • ModificationList: phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive HF

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-01-16 02:28:21ID requested
12019-07-11 00:08:35announced

Publication List

  1. Tian M, Mochizuki K, Loidl J, Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins. Curr Biol, 29(14):2359-2370.e5(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Tetrahymena thermophila, Mediator complex, Emit1, Emit2, Rib1

Contact List

    Josef Loidl
    • contact affiliation: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna BioCenter, Austria
    • contact email: josef.loidl@univie.ac.at
    • lab head:
    Miao Tian
    • contact affiliation: Max F. Perutz Laboratories
    • contact email: tian.miao@univie.ac.at
    • dataset submitter:

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