PXD012335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The synthetic phenotype of bamBbamE double mutants results from a lethal jamming of the Bam complex by the lipoprotein RcsF |
Description | In Gram-negative bacteria, such as Escherichia coli, the heteropentomeric -barrel assembly machine (Bam) folds and inserts proteins into the outer membrane of the cell envelope. Here, we show that the conditional lethal phenotype of a mutant lacking two of the three nonessential lipoproteins, BamB and BamE, is caused by the lethal jamming of the stripped down Bam complex by a normally surface-exposed lipoprotein, RcsF. Our study highlights the importance of the nonessential Bam complex lipoproteins, BamB and BamE, in regulating the interaction between the essential Bam proteins, BamA and BamD and expands our understanding of the role of the Bam complex in outer membrane biogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2019-04-24 |
AnnouncementXML | Submission_2019-05-31_02:38:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Meera Gupta |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-14 07:44:48 | ID requested | |
1 | 2019-04-24 08:51:59 | announced | |
⏵ 2 | 2019-05-31 02:38:55 | announced | Updated publication reference for PubMed record(s): 31113901. |
Publication List
Hart EM, Gupta M, W, ü, hr M, Silhavy TJ, Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF. mBio, 10(3):(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: proteomics, BAM, bacteria, outer membrane |
Contact List
Martin Wuehr |
contact affiliation | Department of Molecular Biology & the Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA |
contact email | wuhr@princeton.edu |
lab head | |
Meera Gupta |
contact affiliation | Princeton University |
contact email | meerag@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012335 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012335
- Label: PRIDE project
- Name: The synthetic phenotype of bamBbamE double mutants results from a lethal jamming of the Bam complex by the lipoprotein RcsF