PXD012335

PXD012335 is an original dataset announced via ProteomeXchange.

Title	The synthetic phenotype of bamBbamE double mutants results from a lethal jamming of the Bam complex by the lipoprotein RcsF
Description	In Gram-negative bacteria, such as Escherichia coli, the heteropentomeric -barrel assembly machine (Bam) folds and inserts proteins into the outer membrane of the cell envelope. Here, we show that the conditional lethal phenotype of a mutant lacking two of the three nonessential lipoproteins, BamB and BamE, is caused by the lethal jamming of the stripped down Bam complex by a normally surface-exposed lipoprotein, RcsF. Our study highlights the importance of the nonessential Bam complex lipoproteins, BamB and BamE, in regulating the interaction between the essential Bam proteins, BamA and BamD and expands our understanding of the role of the Bam complex in outer membrane biogenesis.
HostingRepository	PRIDE
AnnounceDate	2019-04-24
AnnouncementXML	Submission_2019-05-31_02:38:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meera Gupta
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-14 07:44:48	ID requested
1	2019-04-24 08:51:59	announced
⏵ 2	2019-05-31 02:38:55	announced	Updated publication reference for PubMed record(s): 31113901.

Hart EM, Gupta M, W, ü, hr M, Silhavy TJ, Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF. mBio, 10(3):(2019) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, BAM, bacteria, outer membrane

Martin Wuehr
contact affiliation	Department of Molecular Biology & the Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
contact email	wuhr@princeton.edu
lab head
Meera Gupta
contact affiliation	Princeton University
contact email	meerag@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012335
     1. Label: PRIDE project
     2. Name: The synthetic phenotype of bamBbamE double mutants results from a lethal jamming of the Bam complex by the lipoprotein RcsF