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PXD012293

DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-04-03
  • AnnouncementXML: Submission_2019-04-03_10:36:48.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Chris Petzold
  • Title: Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates
  • Description: Background. Many microbial hosts used for the rapid discovery and development of metabolic pathways can have sensitivities to final products and process reagents. Isopentenol, a biogasoline candidate, has an established heterologous gene pathway but is toxic to many microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints. Results. We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum. Isopentenol titers were improved via three routes: media optimization; substitution of an NADH-dependent HmgR homolog from Silicibacter pomeroyi; and development of a C. glutamicum ∆poxB ∆ldhA host chassis.
  • SpeciesList: scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
  • ModificationList: Deamidated; Carbamidomethyl; Oxidation
  • Instrument: 6460 Triple Quadrupole LC/MS

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-01-09 10:47:46ID requested
12019-04-03 10:36:49announced

Publication List

  1. Sasaki Y, Eng T, Herbert RA, Trinh J, Chen Y, Rodriguez A, Gladden J, Simmons BA, Petzold CJ, Mukhopadhyay A, to produce the biogasoline isopentenol from plant biomass hydrolysates. Biotechnol Biofuels, 12():41(2019) [pubmed]

Keyword List

  1. submitter keyword: C. glutamicum, isoprenoid pathway, targeted proteomics

Contact List

    Chris Petzold
    • contact affiliation: Lawrence Berkeley National Laboratory
    • contact email: cjpetzold@lbl.gov
    • lab head:
    Chris Petzold
    • contact affiliation: Lawrence Berkeley National Laboratory
    • contact email: cjpetzold@lbl.gov
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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