<<< Full experiment listing

PXD012293-1

PXD012293 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEngineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates
DescriptionBackground. Many microbial hosts used for the rapid discovery and development of metabolic pathways can have sensitivities to final products and process reagents. Isopentenol, a biogasoline candidate, has an established heterologous gene pathway but is toxic to many microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints. Results. We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum. Isopentenol titers were improved via three routes: media optimization; substitution of an NADH-dependent HmgR homolog from Silicibacter pomeroyi; and development of a C. glutamicum ∆poxB ∆ldhA host chassis.
HostingRepositoryPanoramaPublic
AnnounceDate2019-04-03
AnnouncementXMLSubmission_2019-04-03_10:36:48.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChris Petzold
SpeciesList scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationListDeamidated; Carbamidomethyl; Oxidation
Instrument6460 Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-01-09 10:47:46ID requested
12019-04-03 10:36:49announced
Publication List
Sasaki Y, Eng T, Herbert RA, Trinh J, Chen Y, Rodriguez A, Gladden J, Simmons BA, Petzold CJ, Mukhopadhyay A, to produce the biogasoline isopentenol from plant biomass hydrolysates. Biotechnol Biofuels, 12():41(2019) [pubmed]
Keyword List
submitter keyword: C. glutamicum, isoprenoid pathway, targeted proteomics
Contact List
Chris Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
lab head
Chris Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
Full Dataset Link List
Panorama Public dataset URI