PXD012158

PXD012158 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	proteomics and phosphoprotemics analysis of Ndufs4 KO and wild-type mouse brain upon rapamycin treatment
Description	+ 2 experiments: - effect of rapamycin in Ndufs4 KO mouse --> 3 groups: WT, KO and KR (KO+RP) - effect of rapamycin in wild-type mouse --> 2 groups: WT and WR (WT+RP) + samples from whole brains homogenized in liquid nitrogen + proteins digested with trypsin. No fractionation + phosphopeptides enriched with IMAC beads + instruments: Orbitrap Q Exactive (for proteome), Orbitrap LTQ Velos (for phosphoproteome) + 90 minutes gradient runs + search and quantification with MaxQuant
HostingRepository	MassIVE
AnnounceDate	2020-07-15
AnnouncementXML	Submission_2020-07-15_22:26:28.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Martin Perez
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Phospho
Instrument	Q Exactive; LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-21 07:15:23	ID requested
⏵ 1	2020-07-15 22:26:29	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: brain, mouse, mitochondria, leigh syndrome, rapamycin, signalling, phosphorylation, respiratory complex I, ndufs4, NADH ubiquinone, proteome, phosphoproteome, PKC, mTOR, respiratory chain, inflammation, neurodegeneration

submitter keyword: brain, mouse, mitochondria, leigh syndrome, rapamycin, signalling, phosphorylation, respiratory complex I, ndufs4, NADH ubiquinone, proteome, phosphoproteome, PKC, mTOR, respiratory chain, inflammation, neurodegeneration

Contact List

Judit Villen
contact affiliation	University of Washington
contact email	jvillen@uw.edu
lab head
Miguel Martin Perez
contact affiliation	University of Washington
contact email	mmarper@uw.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083273/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083273/