3 groups: WT, KO and KR (KO+RP) - effect of rapamycin in wild-type mouse --> 2 groups: WT and WR (WT+RP) + samples from whole brains homogenized in liquid nitrogen + proteins digested with trypsin. No fractionation + phosphopeptides enriched with IMAC beads + instruments: Orbitrap Q Exactive (for proteome), Orbitrap LTQ Velos (for phosphoproteome) + 90 minutes gradient runs + search and quantification with MaxQuant]]>