3 groups: WT, KO and KR (KO+RP)
- effect of rapamycin in wild-type mouse --> 2 groups: WT and WR (WT+RP)
+ samples from whole brains homogenized in liquid nitrogen
+ proteins digested with trypsin. No fractionation
+ phosphopeptides enriched with IMAC beads
+ instruments: Orbitrap Q Exactive (for proteome), Orbitrap LTQ Velos (for phosphoproteome)
+ 90 minutes gradient runs
+ search and quantification with MaxQuant]]>