PXD012082 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulation of the endosomal SNX27 retromer by OTULIN |
Description | OTULIN specifically hydrolyzes methionine1 (Met1)-linked ubiquitin chains conjugated by LUBAC (linear ubiquitin chain assembly complex). Using mass spectrometry, we discovered an interaction of OTULIN with SNX27 (sorting nexin 27), an adaptor of the endosomal retromer complex responsible for protein recycling to the cell surface. The C-terminal PDZ binding motif (PDZbm) in OTULIN associates with the cargo-binding site in the PDZ domain of SNX27. By solving the structure of the OTU domain in complex with the PDZ domain, we demonstrate that a second interface contributes to the selective, high affinity interaction of OTULIN and SNX27. SNX27 does not affect OTULIN catalytic activity, OTULIN-LUBAC binding or Met1-linked ubiquitin chain homeostasis. However, via association OTULIN antagonizes SNX27-dependent cargo loading, binding of SNX27 to the retromer subunit VPS26 and endosome-to-membrane trafficking. Thus, we define an additional, non-catalytic function of OTULIN in the regulation of retromer assembly and protein recycling to the cell surface. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-25 |
AnnouncementXML | Submission_2019-09-25_06:03:28.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adan Pinto-Fernandez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-17 06:26:06 | ID requested | |
⏵ 1 | 2019-09-25 06:03:30 | announced | |
Publication List
Stangl A, Elliott PR, Pinto-Fernandez A, Bonham S, Harrison L, Schaub A, Kutzner K, Keusekotten K, Pfluger PT, El Oualid F, Kessler BM, Komander D, Krappmann D, Regulation of the endosomal SNX27-retromer by OTULIN. Nat Commun, 10(1):4320(2019) [pubmed] |
Keyword List
submitter keyword: OTULIN, acitivity-based probe, interactome |
Contact List
Benedikt Kessler |
contact affiliation | TDI-NDM University of Oxford |
contact email | benedikt.kessler@ndm.ox.ac.uk |
lab head | |
Adan Pinto-Fernandez |
contact affiliation | University of Oxford |
contact email | adan.pintofernandez@ndm.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012082
- Label: PRIDE project
- Name: Regulation of the endosomal SNX27 retromer by OTULIN