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PXD011795 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHiRIEF Proteomics of Arabidopsis thaliana Col-0 and CIAF1 mutants
DescriptionThe assembly of the mitochondrial Complex I requires the expression of nuclear and mitochondrial located genes, co-factor biosynthesis and the assembly of at least 44 subunits. This requires the involvement of assembly factors that interact with subunits of Complex I but are not part of the final holocomplex. A novel plant specific mitochondrial matrix protein encoded by At1g76060, named COMPLEX I ASSEMBLY FACTOR 1 (CIAF1), containing a conserved LYR domain, was shown to be required for Complex I activity. T-DNA insertion mutants of At1g76060 lack the monomeric Complex I and the Supercomplex I+III, displaying the common Complex I growth deficient phenotype. Assembly of Complex I is stalled at 650 and 800 kDa intermediates in mitochondria isolated from these mutants. Evidence points to CIAF1 playing an essential role in the assembly of the peripheral matrix arm Complex I subunits to form the holoenzyme Complex I. We here performed quantitative proteomics by HiRIEF LC-MS with a TMT10plex isobaric tag labeling strategy comparing the control plant Col-0 and several mutant lines, including the mutant with inactivating insertion in At1g76060 (a.k.a. Ciaf1-1, a.k.a. SALK_143656). Among up-regulated proteins were components required for mitochondrial biogenesis, which points to a mitochondrial retrograde signaling pathway being activated and executed in response to the lack of Complex I.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRui Branca
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-22 01:49:17ID requested
12019-10-08 01:33:17announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis thaliana, HiRIEF, CIAF1, At1g76060, Col-0, SALK_143656, Tim23, Mitochondria, Complex I
Contact List
Janne Lehtiö
contact affiliationDept. Oncology-Pathology, Science for Life Laboratory, Karolinska Institutet, Sweden
contact emailjanne.lehtio@ki.se
lab head
Rui Branca
contact affiliationClinical Proteomics Unit, Dep. of Oncology-Pathology
contact emailrui.mamede-branca@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List