PXD011789 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mitoprotein-induced stress launches a global transcriptional fail-safe program |
Description | Most mitochondrial proteins are synthesized as cytosolic precursor proteins before being imported into mitochondria. Cytosolic accumulation of mitochondrial precursors hazards cellular fitness and is associated with a growing number of diseases, but is not observed under physiological conditions. Individual mechanisms that allow cells to avoid cytosolic accumulation of mitochondrial precursors have recently been discovered, but their interplay and regulation remain elusive. Here we show that cells rapidly launch a global transcriptional program to restore cellular proteostasis after induction of a “clogger” protein that reduces the number of available mitochondrial import sites. Cells upregulate the protein folding and proteolytic systems in the cytosol and downregulate both the cytosolic translation machinery and many mitochondrial metabolic enzymes, presumably to relieve the workload of the overstrained mitochondrial import system. We show that this transcriptional remodeling is a combination of a “wideband” core response regulated by the transcription factors Hsf1 and Rpn4 and a unique mitoprotein-induced downregulation of the oxidative phosphorylation components, the most abundant group of precursorsmediated by an inactivation of the Hap2/3/4/5 complex. These findings depict the first comprehensive, time-resolved model of adaptations to mitochondrial import impairment. This reveals the regulation of previously described reactions and combines them with a so far unidentified mitochondrion-specific arm into an overarching coordinated network of the cellular mitoprotein-induced stress response. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-14 |
AnnouncementXML | Submission_2019-04-12_00:32:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Felix Boos |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-21 23:54:59 | ID requested | |
1 | 2019-01-13 23:42:27 | announced | |
⏵ 2 | 2019-04-12 00:32:42 | announced | Updated publication reference for PubMed record(s): 30886345. |
Publication List
Boos F, Kr, ä, mer L, Groh C, Jung F, Haberkant P, Stein F, Wollweber F, Gackstatter A, Z, ö, ller E, van der Laan M, Savitski MM, Benes V, Herrmann JM, Mitochondrial protein-induced stress triggers a global adaptive transcriptional programme. Nat Cell Biol, 21(4):442-451(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Mitochondria, Protein Import, Stress Response, Yeast, Proteasome, Chaperones, Oxidative Phosphorylation |
Contact List
Johannes M. Herrmann |
contact affiliation | University of Kaiserlautern Cell Biology |
contact email | hannes.herrmann@biologie.uni-kl.de |
lab head | |
Felix Boos |
contact affiliation | University of Kaiserslautern |
contact email | fboos@rhrk.uni-kl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011789
- Label: PRIDE project
- Name: Mitoprotein-induced stress launches a global transcriptional fail-safe program