PXD011690-2

PXD011690 is an original dataset announced via ProteomeXchange.

Title	Multi-organ systems study reveals Igfbp7 as a suppressor of gluconeogenesis after gastric bypass surgery
Description	Abstract Roux-en-Y gastric bypass (RYGB) surgery reduces weight in obese patients. A marked decrease in blood glucose levels occurs before weight loss; however, key molecules that improve glycemic profile remain largely unknown. Using a murine RYGB surgery model, we performed multi-organ proteomics and bioinformatics to monitor the proteins and molecular pathways that change in this early glycemic response. Multiplexed proteomic kinetics data analysis revealed that the Roux limb, biliopancreatic limb, liver and pancreas each exhibited unique temporal and molecular responses to the RYGB surgery. In addition, protein-protein network analysis indicated that the changes to the microbial environment in the intestine may play a crucial role in the beneficial effects of RYGB surgery. Furthermore, insulin-like growth factor binding protein 7 (Igfbp7) was identified as an early induced protein in the Roux limb. Known secretory properties of Igfbp7 prompted us to further investigate its role as a remote organ regulator of glucose metabolism. Igfbp7 overexpression decreased blood glucose levels in diet-induced obese mice, and attenuated gluconeogenic gene expression in the liver. Secreted Igfbp7 appeared to mediate these beneficial effects. These results demonstrate that organs responded differentially to RYGB surgery, and indicate Igfbp7 may play an important role in improving blood glucose levels.
HostingRepository	PRIDE
AnnounceDate	2019-08-22
AnnouncementXML	Submission_2019-08-26_23:43:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sasha Singh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-13 08:16:16	ID requested
1	2019-08-22 07:12:02	announced
⏵ 2	2019-08-26 23:43:45	announced	Updated publication reference for PubMed record(s): 30848916.
3	2020-01-27 03:57:51	announced	2020-01-27: Updated publication reference for PubMed record(s): 30848916.

Higashi H, Maejima T, Lee LH, Yamazaki Y, Hottiger MO, Singh SA, Aikawa M, -Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation. J Proteome Res, 18(4):1607-1622(2019) [pubmed]

submitter keyword: Bariatric surgery, Metabolic Syndrome, Obesity, Diabetes, Tandem mass tagging, Proteomics, Protein-protein interactions, Insulin-like growth factor binding protein 7

Sasha Singh
contact affiliation	Brigham and Women's Hospital Harvard University
contact email	sasingh@bwh.harvard.edu
lab head
Sasha Singh
contact affiliation	Brigham and Women's Hospital
contact email	sasingh@partners.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011690
     1. Label: PRIDE project
     2. Name: Multi-organ systems study reveals Igfbp7 as a suppressor of gluconeogenesis after gastric bypass surgery