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PXD011667-2

PXD011667 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDirhodium (II) Complex Interferes with Iron-Transport System to Exert Antibacterial Action against Streptococcus pneumoniae
DescriptionDrug resistance in bacteria is becoming a significant threat to global public health, and the development of novel and efficient antibacterial compounds is urgently needed. Recently, rhodium complexes have attracted attention as antimicrobial agents, yet their antibacterial mechanism remains unknown. In this study, we observed that the dirhodium (II) complex Rh2Ac4 inhibited Streptococcus. pneumoniae growth without significant cytotoxic side-effects on host cells in vitro. We subsequently investigated the antibacterial mechanism of Rh2Ac4 using iTRAQ-based proteomics combined with cellular and biochemical assays. Bioinformatics analysis on the proteomic alterations demonstrated that six molecular functional groups, including metal ion binding and twelve metabolic pathways, were significantly affected after treatment with Rh2Ac4. The interaction network analysis of metal ion binding proteins suggested that Rh2Ac4 decreased the protein expression levels of SPD_1652, SPD_1590 and Gap, which are associated with haem uptake/metabolism. Cellular and biochemical assays further confirmed that Rh2Ac4 could be taken up by bacteria via the PiuABCD haem-uptake system. The structurally similar Rh complex may compete with Fe-haem to decrease Fe-uptake via the PiuABCD system, disrupting iron metabolism to exert its antibacterial activity against S. pneumoniae. These data indicate that Rh2Ac4 is a promising new drug for the treatment of S. pneumoniae infections.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:56.505.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYang Xiao-Yan
SpeciesList scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-12 08:28:24ID requested
12022-02-28 02:32:22announced
22023-11-14 08:49:56announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: antibacterial mechanism, Streptococcus pneumoniae, haem-transport system PiuABCD, iTRAQ,Dirhodium (II) complex
Contact List
Qing-Yu He
contact affiliationKey Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan UniversityHealth Engineering, College of Life Science and Technology, Jinan University
contact emailtqyhe@jnu.edu.cn
lab head
Yang Xiao-Yan
contact affiliationJinan University
contact emailouyangxiangyan@126.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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