PXD011626-1

PXD011626 is an original dataset announced via ProteomeXchange.

Title	Molecular profiling of neonatal dried blood spots reveals changes in innate and adaptive immunity following fetal inflammatory response
Description	The fetal inflammatory response (FIR) increases the risk of preterm neonates to adverse neonatal outcomes, such as perinatal brain injury, particularly in extremely low gestational age newborns (ELGANs, < 28 weeks of gestation). One of the mechanisms contributing to such adverse neonatal outcomes is a postnatal intermittent or sustained systemic inflammation (ISSI) following FIR. This link between fetal and neonatal systemic inflammation is supported by the presence of well-established inflammatory biomarkers in umbilical cord (UC) and peripheral blood. However, the extent of molecular changes contributing to this association is unknown. Using RNA sequencing and mass spectrometry proteomics, we have profiled the transcriptome and proteome of archived dried blood spots (DBS) from 21 ELGANs. Comparing ELGANs who were exposed to FIR (n=10) against those that were not (n=11), we identified, respectively, 783 and 27 gene and protein expression changes, with a minimum 50%-fold change and an experiment-wide significance level below 5% false discovery rate. These expression changes confirm the persistent and robust activation of the innate immune system in FIR-affected ELGANs and provide new insights into the molecular mechanisms underlying sustained systemic inflammation and perinatal brain injury. Interestingly, 21 out of the 27 differentially expressed proteins did not match transcriptomic changes, thereby concealing clues on the link between FIR and ISSI that could not be uncovered by either transcriptomics or known biomarkers alone. These findings contribute to the understanding of the association between FIR and ISSI, provide DBS biomarkers of these two systemic inflammatory conditions and may help to identify future therapeutic targets for the associated perinatal disorders.
HostingRepository	PRIDE
AnnounceDate	2019-06-26
AnnouncementXML	Submission_2019-06-26_04:11:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabidó
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-08 03:34:37	ID requested
⏵ 1	2019-06-26 04:11:49	announced
2	2020-12-23 04:53:49	announced	2020-12-23: Updated project metadata.
3	2020-12-23 05:02:16	announced	2020-12-23: Updated project metadata. 2020-12-23: Updated publication reference for PubMed record(s): 33006196.
4	2021-01-04 04:17:57	announced	2021-01-04: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, fetal inflammatory response, dried blood spots

Eduard Sabidó
contact affiliation	Center for Genomic Regulation, Universitat Pompeu Fabra, 08003 Barcelona, Spain
contact email	eduard.sabido@crg.cat
lab head
Eduard Sabidó
contact affiliation	Centre de Regulació Genòmica
contact email	eduard.sabido@crg.cat
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011626
     1. Label: PRIDE project
     2. Name: Molecular profiling of neonatal dried blood spots reveals changes in innate and adaptive immunity following fetal inflammatory response