PXD011487-1
PXD011487 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods |
Description | To evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction method. Furthermore, the P/GTC extracted proteins were analyzed by 2D-LC-MS/MS. |
HostingRepository | jPOST |
AnnounceDate | 2019-10-28 |
AnnouncementXML | Submission_2019-10-27_08:00:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yusuke Kawashima |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-10-27 09:29:21 | ID requested | |
⏵ 1 | 2019-10-27 08:00:09 | announced | |
2 | 2021-02-04 17:02:56 | announced | 2021-02-04: Updated FTP location. |
3 | 2022-09-18 03:28:38 | announced | 2022-09-18: Updated FTP location. |
Publication List
Kawashima Y, Miyata J, Watanabe T, Shioya J, Arita M, Ohara O, Proteogenomic Analyses of Cellular Lysates Using a Phenol-Guanidinium Thiocyanate Reagent. J Proteome Res, 18(1):301-308(2019) [pubmed] |
Keyword List
submitter keyword: HEK293 cell, neutrophil, phenol-guanidinium isothiocyanate |
Contact List
Yusuke Kawashima | |
---|---|
lab head | |
Yusuke Kawashima | |
contact affiliation | Kazusa DNA Research Institute |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000513 |