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PXD011487

PXD011487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods
DescriptionTo evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction method. Furthermore, the P/GTC extracted proteins were analyzed by 2D-LC-MS/MS.
HostingRepositoryjPOST
AnnounceDate2019-10-28
AnnouncementXMLSubmission_2022-09-18_03:28:37.606.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYusuke Kawashima
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-27 09:29:21ID requested
12019-10-27 08:00:09announced
22021-02-04 17:02:56announced2021-02-04: Updated FTP location.
32022-09-18 03:28:38announced2022-09-18: Updated FTP location.
Publication List
Kawashima Y, Miyata J, Watanabe T, Shioya J, Arita M, Ohara O, Proteogenomic Analyses of Cellular Lysates Using a Phenol-Guanidinium Thiocyanate Reagent. J Proteome Res, 18(1):301-308(2019) [pubmed]
Keyword List
submitter keyword: HEK293 cell, neutrophil, phenol-guanidinium isothiocyanate
Contact List
Yusuke Kawashima
lab head
Yusuke Kawashima
contact affiliationKazusa DNA Research Institute
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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