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PXD011223-2

PXD011223 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGené organ engagement in lipid metabolism revealed by a combined transcriptomic and proteomic approach
DescriptionGené organ (GO) is an exclusive apparatus of female ticks that secretes a protective wax coat onto the egg surface. This wax coat makes eggs more viable in the environment due to its waterproof, stickiness and antimicrobial proprieties. In this work, a combined transcriptomic and proteomic approach shows that GO is engaged in synthesis, modification and oxidation of lipid compounds, not solely secrets compounds uptaked from haemolymph onto the egg surface. GO were analysed at two distinct stages: (i) in the day haematophagy is concluded and ticks detach from host and (ii) during egg laying, precisely at the second day of oviposition. Data presented here show that before oviposition begins, GO undergoes a maturing process in order to be ready to play its role during oviposition. Before oviposition, GO content is directed to machineries of cell division, transcription and protein synthesis, including proteins participating in the cytoskeleton structure. Whereas during egg laying, the content of proteins related to secretion, transporting and metabolism of wax is overrepresented, though protein synthesis is also preserved. Since GO is a wax secretion organ, the work was focused on lipid metabolism, examining a full machinery to synthesize, modify and oxidize fatty acids. In addition, proteins involved on sterols modification, transport and degradation were addressed. Further than highlighting GO importance in tick reproduction physiology, data reveals molecules and pathways crucial to egg wax secretion, and consequently, its development in the environment. Tools targeting these molecules and pathways would impair egg development in environment, and so could be potential targets for novel tick control methods.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:55.362.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterItabajara Vaz Jr.
SpeciesList scientific name: Rhipicephalus microplus; NCBI TaxID: 6941;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-28 07:49:18ID requested
12022-03-01 08:32:34announced
22023-11-14 08:49:55announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: transcriptome,Gené organ, lipid gland, tick, egg wax, proteome
Contact List
Itabajara da Silva Vaz Junior
contact affiliationFaculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
contact emailitabajara.vaz@ufrgs.br
lab head
Itabajara Vaz Jr.
contact affiliationUniversidade Federal do Rio Grande do Sul
contact emailitabajara.vaz@ufrgs.br
dataset submitter
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