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PXD011211-2

PXD011211 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly
DescriptionTFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique subunit composition exist and several TFIID subunits are shared with other complexes, conveying intricate cellular decision making to control subunit allocation and functional assembly of this essential transcription factor. However, the underlying molecular mechanisms remain poorly understood. Here, we used quantitative proteomics to examine TFIID submodules and assembly mechanisms in human cells. Structural and mutational analysis of the cytoplasmic TAF5/TAF6/TAF9 submodule identified novel interactions crucial for TFIID integrity, and for allocating TAF9 to TFIID or the SAGA co-activator complex. We discover a key checkpoint function for the chaperonin CCT, which specifically associates with nascent TAF5 for subsequent handover to TAF6/TAF9 and ultimate holo-TFIID formation. Our findings illustrate at the molecular level how multisubunit complexes are crafted in the cell, involving checkpoint decisions facilitated by a chaperone machine.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:36.624.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimona Antonova
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-27 11:32:02ID requested
12022-03-02 07:12:58announced
22023-11-14 08:49:37announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomics , crystal structure, TFIID, chaperonin CCT
Contact List
Marc Timmers
contact affiliationProfessor of Medical Epigenetics German Cancer Consortium (DKTK), partner site Freiburg German Cancer Research Center (DKFZ) Foundation under Public Law c/o Zentrale Klinische Forschung (ZKF) University Medical Center Freiburg Breisacher Str. 66, 79106 Freiburg, Germany
contact emailm.timmers@dkfz-heidelberg.de
lab head
Simona Antonova
contact affiliationRMCU, University Medical Center, Utrecht
contact emails.v.antonova-2@umcutrecht.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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