PXD011201-1

PXD011201 is an original dataset announced via ProteomeXchange.

Title	A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation
Description	Small RNA silencing pathways protect genome integrity in part through establishing heterochromatin at transposon loci. In animals, this process requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts. The molecular events contributing to heterochromatin formation upon PIWI binding to nascent RNA, a transient molecule at chromatin, are unknown. Here, we identify SFINX, a protein complex that is required for Piwi-mediated co-transcriptional silencing in Drosophila. It consists of Nxf2—a variant of the nuclear RNA export factor Nxf1/Tap, the mRNA export co-factor Nxt1/p15, and the Piwi-associated protein Panoramix. In the absence of Nxf2, Panoramix is targeted for degradation and piRNA-loaded Piwi is unable to establish heterochromatin. Consequently, nxf2 mutants exhibit severe transposon de-repression and are sterile. We show that within SFINX, Panoramix connects to the heterochromatin machinery while Nxf2 enables target silencing via nascent RNA. Thus, the Nxf2-Nxt1 heterodimer—despite having originated from core mRNA export machinery—has been repurposed for heterochromatin formation. Our data establish an unexpected link between nuclear small RNA biology and NXF-variants, which are widespread in animal lineages, but mostly lack ascribed functions.
HostingRepository	PRIDE
AnnounceDate	2019-06-12
AnnouncementXML	Submission_2019-06-12_04:57:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-27 05:52:55	ID requested
⏵ 1	2019-06-12 04:57:53	announced
2	2019-08-16 00:25:42	announced	Updated publication reference for PubMed record(s): 31384064.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Panoramix, Silencio, Nxf2, Nxt1, SFINX, OSC

Julius Fabian Brennecke
contact affiliation	IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
contact email	julius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011201
     1. Label: PRIDE project
     2. Name: A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation