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PXD011201

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-06-12
  • AnnouncementXML: Submission_2019-08-16_00:25:40.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Richard Imre
  • Title: A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation
  • Description: Small RNA silencing pathways protect genome integrity in part through establishing heterochromatin at transposon loci. In animals, this process requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts. The molecular events contributing to heterochromatin formation upon PIWI binding to nascent RNA, a transient molecule at chromatin, are unknown. Here, we identify SFINX, a protein complex that is required for Piwi-mediated co-transcriptional silencing in Drosophila. It consists of Nxf2—a variant of the nuclear RNA export factor Nxf1/Tap, the mRNA export co-factor Nxt1/p15, and the Piwi-associated protein Panoramix. In the absence of Nxf2, Panoramix is targeted for degradation and piRNA-loaded Piwi is unable to establish heterochromatin. Consequently, nxf2 mutants exhibit severe transposon de-repression and are sterile. We show that within SFINX, Panoramix connects to the heterochromatin machinery while Nxf2 enables target silencing via nascent RNA. Thus, the Nxf2-Nxt1 heterodimer—despite having originated from core mRNA export machinery—has been repurposed for heterochromatin formation. Our data establish an unexpected link between nuclear small RNA biology and NXF-variants, which are widespread in animal lineages, but mostly lack ascribed functions.
  • SpeciesList: scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
  • ModificationList: methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-09-27 05:52:55ID requested
12019-06-12 04:57:53announced
22019-08-16 00:25:42announcedUpdated publication reference for PubMed record(s): 31384064.

Publication List

  1. Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J, The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol, 26(8):720-731(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Panoramix, Silencio, Nxf2, Nxt1, SFINX, OSC

Contact List

    Julius Fabian Brennecke
    • contact affiliation: IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
    • contact email: julius.brennecke@imba.oeaw.ac.at
    • lab head:
    Richard Imre
    • contact affiliation: IMBA - Institute of Molecular Biotechnology
    • contact email: imre@imp.ac.at
    • dataset submitter:

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