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PXD011201 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation
DescriptionSmall RNA silencing pathways protect genome integrity in part through establishing heterochromatin at transposon loci. In animals, this process requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts. The molecular events contributing to heterochromatin formation upon PIWI binding to nascent RNA, a transient molecule at chromatin, are unknown. Here, we identify SFINX, a protein complex that is required for Piwi-mediated co-transcriptional silencing in Drosophila. It consists of Nxf2—a variant of the nuclear RNA export factor Nxf1/Tap, the mRNA export co-factor Nxt1/p15, and the Piwi-associated protein Panoramix. In the absence of Nxf2, Panoramix is targeted for degradation and piRNA-loaded Piwi is unable to establish heterochromatin. Consequently, nxf2 mutants exhibit severe transposon de-repression and are sterile. We show that within SFINX, Panoramix connects to the heterochromatin machinery while Nxf2 enables target silencing via nascent RNA. Thus, the Nxf2-Nxt1 heterodimer—despite having originated from core mRNA export machinery—has been repurposed for heterochromatin formation. Our data establish an unexpected link between nuclear small RNA biology and NXF-variants, which are widespread in animal lineages, but mostly lack ascribed functions.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRichard Imre
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmethylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-27 05:52:55ID requested
12019-06-12 04:57:53announced
22019-08-16 00:25:42announcedUpdated publication reference for PubMed record(s): 31384064.
Publication List
Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J, The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol, 26(8):720-731(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Panoramix, Silencio, Nxf2, Nxt1, SFINX, OSC
Contact List
Julius Fabian Brennecke
contact affiliationIMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
contact emailjulius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliationIMBA - Institute of Molecular Biotechnology
contact emailimre@imp.ac.at
dataset submitter
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