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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-06-12
  • AnnouncementXML: Submission_2019-06-12_04:57:52.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Richard Imre
  • Title: A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation
  • Description: Small RNA silencing pathways protect genome integrity in part through establishing heterochromatin at transposon loci. In animals, this process requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts. The molecular events contributing to heterochromatin formation upon PIWI binding to nascent RNA, a transient molecule at chromatin, are unknown. Here, we identify SFINX, a protein complex that is required for Piwi-mediated co-transcriptional silencing in Drosophila. It consists of Nxf2—a variant of the nuclear RNA export factor Nxf1/Tap, the mRNA export co-factor Nxt1/p15, and the Piwi-associated protein Panoramix. In the absence of Nxf2, Panoramix is targeted for degradation and piRNA-loaded Piwi is unable to establish heterochromatin. Consequently, nxf2 mutants exhibit severe transposon de-repression and are sterile. We show that within SFINX, Panoramix connects to the heterochromatin machinery while Nxf2 enables target silencing via nascent RNA. Thus, the Nxf2-Nxt1 heterodimer—despite having originated from core mRNA export machinery—has been repurposed for heterochromatin formation. Our data establish an unexpected link between nuclear small RNA biology and NXF-variants, which are widespread in animal lineages, but mostly lack ascribed functions.
  • SpeciesList: scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
  • ModificationList: methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-09-27 05:52:55ID requested
12019-06-12 04:57:53announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Panoramix, Silencio, Nxf2, Nxt1, SFINX, OSC

Contact List

    Julius Fabian Brennecke
    • contact affiliation: IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
    • contact email: julius.brennecke@imba.oeaw.ac.at
    • lab head:
    Richard Imre
    • contact affiliation: IMBA - Institute of Molecular Biotechnology
    • contact email: imre@imp.ac.at
    • dataset submitter:

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