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PXD011063-1

PXD011063 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA method for Sensitive Quantitative O-Glycoproteomics by Data Independent Acquisition with In Silico expansion of O-Glycopeptide Libraries – Glyco-DIA
DescriptionWe report a LC-MS/MS O-glycoproteomics strategy using Data Independent Acquisition (DIA) mode that holds the potential for enabling direct analysis of O-glycoproteins with characterization of sites and structures of O-glycans on a proteome-wide scale with quantification of stoichiometries. To explore the use of a DIA strategy for O-glycoproteomics, we built a spectral library of O-glycopeptides with the most common core1 O-glycan structures. This Glyco-DIA library consists of sublibraries obtained from cell lines and human serum, and it currently covers 2,076 O-glycoproteins (11,452 unique glycopeptide sequences) and the five most common core1 O-glycan structures. Applying the Glyco-DIA library to human serum without enrichment for glycopeptides enabled us to identify and quantify nearly 293 distinct glycopeptide sequences bearing up to 5 different core1 O-glycans from 159 glycoproteins in a singleshot analysis. The DIA method is expandable and widely applicable to different glycoproteomes, and it may represent the first direct and comprehensive approach to glycoproteomics
HostingRepositoryPRIDE
AnnounceDate2019-08-06
AnnouncementXMLSubmission_2019-08-06_02:45:22.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSergey Vakhrushev
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-11 08:50:41ID requested
12019-08-06 02:45:22announced
22019-08-08 03:21:15announcedUpdated publication reference for PubMed record(s): 31384044.
32019-11-27 00:30:21announced2019-11-27: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Glycoproteomics, Data Independent Acquisition, mass spectrometry, O-glycosylation, human serum
Contact List
Sergey Vakhrushev
contact affiliationCopenhagen Center for Glycomics, University of Copenhagen
contact emailseva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliationDepartment of Cellular and Molecular Medicine
contact emailseva@sund.ku.dk
dataset submitter
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Dataset FTP location
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