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PXD010996-1

PXD010996 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Covalent Modifications Regulating Immune Signaling Complex Composition and Phenotype
DescriptionCells signal through rearrangements of protein communities governed by covalent modifications and reversible interactions of distinct sets of proteins. A method that identifies those post-transcriptional modifications regulating signaling complex composition and functional phenotypes in one experimental setup would facilitate an efficient identification of novel molecular signaling checkpoints. Here we devised Modifications, Interactions and Phenotypes by Affinity Purification Mass Spectrometry (MIP-APMS), comprising the streamlined cloning and transduction of tagged proteins into functionalized reporter cells as well as affinity chromatography, followed by MS-based quantification. We report the time-resolved interplay of more than 50 previously undescribed modification and hundreds of protein-protein interactions of 19 immune protein complexes in monocytes. Validation of interdependecies between covalent, reversible and functional protein complex regulations by knock-out or site-specific mutation, revealed isgylation and phosphorylation of TRAF2 as well as ARHGEF18 interaction in Toll-like receptor 2 signaling. Moreover, we identify distinct mechanisms of action for small molecule inhibitors of p-38 (MAPK14). Our method provides a fast and cost-effective pipeline for the molecular interrogation of protein communities in diverse biological systems and primary cells.
HostingRepositoryPRIDE
AnnounceDate2021-07-07
AnnouncementXMLSubmission_2021-07-06_23:14:53.770.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-04 02:10:07ID requested
12021-07-06 23:14:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, monocytes, AP-MS, TLR2, Phosphorylation, Acetylation, Methylation
Contact List
Felix Meissner
contact affiliationExperimental Systems Immunology, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact emailmeissner@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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