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PXD010411-1

PXD010411 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAcetylation of p73 in 293T cell
Description293T cells ectopically expressing FLAG-tagged p73 were treated with AGK2 and p73 was immunoprecipitated. The precipitants were resuspended in deacetylation buffer (50 mM Tris-HCl pH 8.0, 140 mM NaCl, 2 mM MgCl 2 ) and incubated with recombinant SIRT2 protein (10 U). The reaction was terminated by boiling with SDS sample buffer and acetylation levels were determined by immunoblotting.
HostingRepositoryjPOST
AnnounceDate2018-09-10
AnnouncementXMLSubmission_2018-09-09_18:19:31.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLumi Negishi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-12 23:37:59ID requested
12018-09-09 18:19:32announced
22018-09-19 01:03:49announcedUpdated CV, FTP location and Modification list.
32020-04-07 23:37:20announced2020-04-08: Updated PubMed.
42021-02-04 17:05:56announced2021-02-04: Updated FTP location.
52022-09-18 03:23:50announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: p73, Acetylation, SIRT2, 293T
Contact List
Tetsu Akiyama
lab head
Lumi Negishi
contact affiliationthe University of Tokyo
dataset submitter
Full Dataset Link List
jPOST dataset URI