PXD010411

PXD010411 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Acetylation of p73 in 293T cell
Description	293T cells ectopically expressing FLAG-tagged p73 were treated with AGK2 and p73 was immunoprecipitated. The precipitants were resuspended in deacetylation buffer (50 mM Tris-HCl pH 8.0, 140 mM NaCl, 2 mM MgCl 2 ) and incubated with recombinant SIRT2 protein (10 U). The reaction was terminated by boiling with SDS sample buffer and acetylation levels were determined by immunoblotting.
HostingRepository	jPOST
AnnounceDate	2018-09-10
AnnouncementXML	Submission_2022-09-18_03:23:49.452.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-12 23:37:59	ID requested
1	2018-09-09 18:19:32	announced
2	2018-09-19 01:03:49	announced	Updated CV, FTP location and Modification list.
3	2020-04-07 23:37:20	announced	2020-04-08: Updated PubMed.
4	2021-02-04 17:05:56	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 03:23:50	announced	2022-09-18: Updated FTP location.

Publication List

Funato K, Hayashi T, Echizen K, Negishi L, Shimizu N, Koyama-Nasu R, Nasu-Nishimura Y, Morishita Y, Tabar V, Todo T, Ino Y, Mukasa A, Saito N, Akiyama T, SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep, 19(11):(2018) [pubmed]

Funato K, Hayashi T, Echizen K, Negishi L, Shimizu N, Koyama-Nasu R, Nasu-Nishimura Y, Morishita Y, Tabar V, Todo T, Ino Y, Mukasa A, Saito N, Akiyama T, SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity. EMBO Rep, 19(11):(2018) [pubmed]

Keyword List

submitter keyword: p73, Acetylation, SIRT2, 293T

submitter keyword: p73, Acetylation, SIRT2, 293T

Contact List

Tetsu Akiyama
lab head
Lumi Negishi
contact affiliation	the University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000454/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000454/