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PXD010236-1

PXD010236 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTime-Course Mass Spectrometry Data of Adipose Mesenchymal Stem Cells Acquiring Chondrogenic Phenotype
DescriptionProject description: Adipose mesenchymal stem cells (ADSCs) are fast gaining popularity in regenerative medicine for their pluripotency and relative use of collection. Currently, we are addressing the use of ADSCs in otolaryngology, for the treatment of vocal fold (VF) paralysis. VF can result from accidentally sectioning the recurrent laryngeal nerve (RLN) during thyroidectomy. This prevents closure of the upper larynx and promotes food aspiration into the lungs. Volume augmenting materials are sometimes injected in order to bring the paralyzed VF in close proximity to the counter lateral VF. The procedure is called injection medialization laryngoplasty (IML); we have recently developed a rabbit IML model for testing biological reagents in order to improve outcomes. As part of these studies ADSCs were differentiated into chondrocytes and their fate was studied in vitro and in situ. We used mass spectrometry proteomics because microarrays based gene expression platforms do not report actual protein levels. The data show protein levels in controls and chondrogenically differentiating rabbit ADSCs at 0, 14 and 28 days, analyzed using nano-flow liquid chromatography electrospray ionization tandem mass spectrometry (nLC-MS/MS).
HostingRepositoryMassIVE
AnnounceDate2018-07-12
AnnouncementXMLSubmission_2018-07-12_10:02:22.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSerban San-MArina
SpeciesList scientific name: Oryctolagus cuniculus; common name: rabbit; NCBI TaxID: 9986;
ModificationListunknown modification: Carbamidomethylation of cysteine and oxidation of methionine were allowed as variable modifications
Instrumentinstrument model: Thermo Scientific Q-Exactive Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to a Thermo Ultimate 3000 RSLC nano HPLC system
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-26 13:06:44ID requested
12018-07-12 10:02:22announced
Publication List
no publication
Keyword List
submitter keyword: Proteomics, mass spectrometry, adipose mesenchymal stem cells, regenerative medicine, chondrogenic time-course
Contact List
Serban San-Marina
contact affiliationMayo, Rochester, MN
contact emailsanmarina.serban@mayo.edu
lab head
Serban San-MArina
contact affiliationMayo Clinic Rochester
contact emailsanmarina.serban@mayo.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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