PXD010197 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Farnesylated Heat Shock Protein 40 is a component of membrane-bound RISC in Arabidopsis |
Description | ARGONAUTE1 (AGO1) binds directly to small regulatory RNA and is a key effector protein of post-transcriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). The formation of an RNA induced silencing complex (RISC) of AGO1 and small RNA requires the function of the Heat Shock Protein 70/90 chaperone system. Some functions of AGO1 occur in association with endomembranes, in particular the rough endoplasmic reticulum (rER), but proteins interacting with AGO1 in membrane fractions remain unidentified. In this study, we show that the farnesylated Heat Shock Protein 40 homologs, J2 and J3, associate with AGO1 in membrane fractions in a manner that involves protein farnesylation. We also show that three changes in AGO1 function are detectable in mutants in protein farnesylation and J2/J3. First, perturbations of the HSP40/70/90 pathway by mutation of j3, hsp90 and farnesyl transferase affect the amounts of AGO1 associated with membranes. Second, miRNA association with membrane-bound AGO1, and with membrane-bound polysomes is increased in farnesyl transferase and farnesylationdeficient J2/J3 mutants. Third, silencing by non-cell autonomously acting short interfering RNAs (siRNAs) is impaired. These observations highlight the involvement of farnesylated J2/J3 in small RNA-mediated gene regulation, and suggest that the importance of chaperone-AGO1 interaction is not limited to the RISC assembly process. |
HostingRepository | PRIDE |
AnnounceDate | 2018-09-10 |
AnnouncementXML | Submission_2019-02-12_08:25:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Franziska Voellmy |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-22 05:44:01 | ID requested | |
1 | 2018-09-10 03:04:06 | announced | |
⏵ 2 | 2019-02-12 08:25:26 | announced | Updated publication reference for PubMed record(s): 30194279. |
Publication List
Sj, ö, gren L, Floris M, Barghetti A, V, ö, llmy F, Linding R, Brodersen P, . J Biol Chem, 293(43):16608-16622(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Small RNA, ARGONAUTE, chaperones, farnesylation, rough ER, polysomes. |
Contact List
Peter Brodersen |
contact affiliation | Peter Brodersen, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark |
contact email | pbrodersen@bio.ku.dk |
lab head | |
Franziska Voellmy |
contact affiliation | Utrecht University |
contact email | franziska.voellmy@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010197
- Label: PRIDE project
- Name: Farnesylated Heat Shock Protein 40 is a component of membrane-bound RISC in Arabidopsis