PXD010197

PXD010197 is an original dataset announced via ProteomeXchange.

Title	Farnesylated Heat Shock Protein 40 is a component of membrane-bound RISC in Arabidopsis
Description	ARGONAUTE1 (AGO1) binds directly to small regulatory RNA and is a key effector protein of post-transcriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). The formation of an RNA induced silencing complex (RISC) of AGO1 and small RNA requires the function of the Heat Shock Protein 70/90 chaperone system. Some functions of AGO1 occur in association with endomembranes, in particular the rough endoplasmic reticulum (rER), but proteins interacting with AGO1 in membrane fractions remain unidentified. In this study, we show that the farnesylated Heat Shock Protein 40 homologs, J2 and J3, associate with AGO1 in membrane fractions in a manner that involves protein farnesylation. We also show that three changes in AGO1 function are detectable in mutants in protein farnesylation and J2/J3. First, perturbations of the HSP40/70/90 pathway by mutation of j3, hsp90 and farnesyl transferase affect the amounts of AGO1 associated with membranes. Second, miRNA association with membrane-bound AGO1, and with membrane-bound polysomes is increased in farnesyl transferase and farnesylationdeficient J2/J3 mutants. Third, silencing by non-cell autonomously acting short interfering RNAs (siRNAs) is impaired. These observations highlight the involvement of farnesylated J2/J3 in small RNA-mediated gene regulation, and suggest that the importance of chaperone-AGO1 interaction is not limited to the RISC assembly process.
HostingRepository	PRIDE
AnnounceDate	2018-09-10
AnnouncementXML	Submission_2019-02-12_08:25:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franziska Voellmy
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-22 05:44:01	ID requested
1	2018-09-10 03:04:06	announced
⏵ 2	2019-02-12 08:25:26	announced	Updated publication reference for PubMed record(s): 30194279.

Sj, ö, gren L, Floris M, Barghetti A, V, ö, llmy F, Linding R, Brodersen P, . J Biol Chem, 293(43):16608-16622(2018) [pubmed]

curator keyword: Biological

submitter keyword: Small RNA, ARGONAUTE, chaperones, farnesylation, rough ER, polysomes.

Peter Brodersen
contact affiliation	Peter Brodersen, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK-2200 Copenhagen N, Denmark
contact email	pbrodersen@bio.ku.dk
lab head
Franziska Voellmy
contact affiliation	Utrecht University
contact email	franziska.voellmy@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010197
     1. Label: PRIDE project
     2. Name: Farnesylated Heat Shock Protein 40 is a component of membrane-bound RISC in Arabidopsis