PXD009939 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila |
Description | Stem cells establish cortical polarity and divide asymmetrically to simultaneously maintain themselves and generate differentiating offspring cells. Several chromatin modifiers have been identified as stemness factors in mammalian pluripotent stem cells, but whether these factors control stem cell polarity and asymmetric division has not been investigated so far. We addressed this question in Drosophila neural stem cells called neuroblasts. We identified the Tip60 chromatin remodeling complex and its interaction partner Myc to regulate target genes required for neuroblast maintenance. Knockdown of members of this complex results in loss of cortical polarity, symmetric neuroblast division and premature differentiation through nuclear entry of the transcription factor Prospero. We found that aPKC is the key target gene of Myc and the Tip60 complex subunit Domino regulating neuroblast polarity. Our transcriptome analysis further showed that Domino regulates the expression of mitotic spindle genes which were identified before as direct Myc targets. Our findings reveal an evolutionarily conserved functional link between Myc, the Tip60 complex and the molecular network controlling cell polarity and asymmetric cell division. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-06 |
AnnouncementXML | Submission_2018-07-06_05:39:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Frese |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-28 07:28:35 | ID requested | |
⏵ 1 | 2018-07-06 05:39:43 | announced | |
2 | 2019-06-14 07:40:49 | announced | Updated publication reference for PubMed record(s): 29997178. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: neuroblast, polarity, chromatin remodeling, stem cell, asymmetric cell division |
Contact List
Andreas Wodarz |
contact affiliation | Institut I für Anatomie AG Molekulare Zellbiologie Universität zu Köln Kerpener Str. 62, Geb. 37 D-50937 Cologne Germany |
contact email | andreas.wodarz@uk-koeln.de |
lab head | |
Christian Frese |
contact affiliation | CECAD Research Center Cologne |
contact email | cfrese@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD009939
- Label: PRIDE project
- Name: Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila