PXD009939

PXD009939 is an original dataset announced via ProteomeXchange.

Title	Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila
Description	Stem cells establish cortical polarity and divide asymmetrically to simultaneously maintain themselves and generate differentiating offspring cells. Several chromatin modifiers have been identified as stemness factors in mammalian pluripotent stem cells, but whether these factors control stem cell polarity and asymmetric division has not been investigated so far. We addressed this question in Drosophila neural stem cells called neuroblasts. We identified the Tip60 chromatin remodeling complex and its interaction partner Myc to regulate target genes required for neuroblast maintenance. Knockdown of members of this complex results in loss of cortical polarity, symmetric neuroblast division and premature differentiation through nuclear entry of the transcription factor Prospero. We found that aPKC is the key target gene of Myc and the Tip60 complex subunit Domino regulating neuroblast polarity. Our transcriptome analysis further showed that Domino regulates the expression of mitotic spindle genes which were identified before as direct Myc targets. Our findings reveal an evolutionarily conserved functional link between Myc, the Tip60 complex and the molecular network controlling cell polarity and asymmetric cell division.
HostingRepository	PRIDE
AnnounceDate	2018-07-06
AnnouncementXML	Submission_2019-06-14_07:40:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-28 07:28:35	ID requested
1	2018-07-06 05:39:43	announced
⏵ 2	2019-06-14 07:40:49	announced	Updated publication reference for PubMed record(s): 29997178.

Rust K, Tiwari MD, Mishra VK, Grawe F, Wodarz A, . EMBO J, 37(16):(2018) [pubmed]

curator keyword: Biological

submitter keyword: neuroblast, polarity, chromatin remodeling, stem cell, asymmetric cell division

Andreas Wodarz
contact affiliation	Institut I für Anatomie AG Molekulare Zellbiologie Universität zu Köln Kerpener Str. 62, Geb. 37 D-50937 Cologne Germany
contact email	andreas.wodarz@uk-koeln.de
lab head
Christian Frese
contact affiliation	CECAD Research Center Cologne
contact email	cfrese@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009939

PRIDE project URI

• PRIDE
  1. PXD009939
     1. Label: PRIDE project
     2. Name: Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila