PXD009911 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Soybean root LC-MSMS - iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae |
Description | Soybean (Glycine max) is susceptible to root rot when subjected to continuous cropping, and this disease can seriously diminish the crop yield. Herein, isobaric tag for relative and absolute quantitation (iTRAQ) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were employed for proteomic analysis of continuously cropped soybean inoculated with the arbuscular mycorrhizal (AM) fungus Funneliformis mosseae. Differential expression of proteins in soybean roots was determined following 1 year of continuous cropping. A total of 131 differentially expressed proteins (DEPs) were identified in F. mosseae-treated samples, of which 49 and 82 were up- and down-regulated, respectively. The DEPs were annotated with 117 Gene Ontology (GO) terms, with 48 involved in biological processes, 31 linked to molecular functions, and 39 associated with cell components. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis mapped the DEPs to 113 mainly metabolic pathways including oxidative phosphorylation, glycolysis and amino acid metabolism. Expression of glucan 1,3-beta-glucosidase, chalcone isomerase, calcium-dependent phospholipid binding and other defense-related proteins was up-regulated by F. mosseae, suggesting inoculation promotes the growth and development of soybean and increases disease resistance. The findings provide an experimental basis for further research on the molecular mechanisms of AM fungi in resolving problems associated with continuous soybean cropping. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-04 |
AnnouncementXML | Submission_2021-03-04_12:45:56.552.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | baiyan Cai |
SpeciesList | scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-25 06:21:40 | ID requested | |
⏵ 1 | 2021-03-04 12:45:57 | announced | |
Publication List
Bai L, Sun HB, Liang RT, Cai BY, . Front Microbiol, 10():61(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Soybean root, iTRAQ proteomic analysis |
LC-MS/MS |
Contact List
Baiyan Cai |
contact affiliation | College of Life sciences, Heilongjiang University/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, China |
contact email | caibaiyan@126.com |
lab head | |
baiyan Cai |
contact affiliation | Heilongjiang University |
contact email | caibaiyan@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009911
- Label: PRIDE project
- Name: Soybean root LC-MSMS - iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae