PXD009911

PXD009911 is an original dataset announced via ProteomeXchange.

Title	Soybean root LC-MSMS - iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae
Description	Soybean (Glycine max) is susceptible to root rot when subjected to continuous cropping, and this disease can seriously diminish the crop yield. Herein, isobaric tag for relative and absolute quantitation (iTRAQ) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were employed for proteomic analysis of continuously cropped soybean inoculated with the arbuscular mycorrhizal (AM) fungus Funneliformis mosseae. Differential expression of proteins in soybean roots was determined following 1 year of continuous cropping. A total of 131 differentially expressed proteins (DEPs) were identified in F. mosseae-treated samples, of which 49 and 82 were up- and down-regulated, respectively. The DEPs were annotated with 117 Gene Ontology (GO) terms, with 48 involved in biological processes, 31 linked to molecular functions, and 39 associated with cell components. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis mapped the DEPs to 113 mainly metabolic pathways including oxidative phosphorylation, glycolysis and amino acid metabolism. Expression of glucan 1,3-beta-glucosidase, chalcone isomerase, calcium-dependent phospholipid binding and other defense-related proteins was up-regulated by F. mosseae, suggesting inoculation promotes the growth and development of soybean and increases disease resistance. The findings provide an experimental basis for further research on the molecular mechanisms of AM fungi in resolving problems associated with continuous soybean cropping.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:45:56.552.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	baiyan Cai
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-25 06:21:40	ID requested
⏵ 1	2021-03-04 12:45:57	announced

Bai L, Sun HB, Liang RT, Cai BY, . Front Microbiol, 10():61(2019) [pubmed]

curator keyword: Biological

submitter keyword: Soybean root, iTRAQ proteomic analysis

LC-MS/MS

Baiyan Cai
contact affiliation	College of Life sciences, Heilongjiang University/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, China
contact email	caibaiyan@126.com
lab head
baiyan Cai
contact affiliation	Heilongjiang University
contact email	caibaiyan@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009911
     1. Label: PRIDE project
     2. Name: Soybean root LC-MSMS - iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae